Chk1 s345
WebJun 21, 2004 · Chk1 P-S345 and Chk2 P-T68 antibodies were obtained from Cell Signaling Technology (Beverly, MA). P-S1981 ATM antibody was from Rockland (Gilbertsville, PA). In Vitro Kinase Assays. Kinase assays were performed with Flag-tagged recombinant ATM or ATR proteins produced in human embryonic kidney (HEK) 293 cells as described in ref. 28. WebIn siGFP-transfected cells, p-Chk1 (S345) and p- Cdk2 (T14, Y15) were detected, indicating that HU treatment induced cell cycle arrest ( Figure 4C). p-Chk1 and p-Cdk2 levels were lower in HU ...
Chk1 s345
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WebDec 30, 2008 · Targeting Chk1 Phosphorylation Sites in Human Cells. To assess the functional roles of the S317 and S345 Chk1 phosphorylation sites in human cells, we used a knockin/knockout approach to alter endogenous CHK1 alleles (Fig. 1 A).This strategy was applied to the colorectal cancer cell line DLD-1, which has been extensively used for cell …
WebJun 15, 2000 · We show that in human cells, Chk1 is phosphorylated on serine 345 (S345) in response to UV, IR, and hydroxyurea (HU). Overexpression of wild-type Atr enhances, … WebApr 29, 2009 · S. pombe Chk1 domain structure.Chk1 has an N-terminal kinase domain (blue) and a C-terminal regulatory domain (yellow). Regions required for 14-3-3 interaction and nuclear localization [] are indicated below the schematic.The position of S345 (orange), the site of activating phosphorylation is shown, as are the two regions of the C-terminal …
WebWe propose that Atr is a major regulator of Chk1 in response to DNA damage based on the following observations: (1) Chk1 phosphorylation on S345 is increased in response to … Phospho-Chk1 (Ser345) Antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345. This antibody does not cross-react with Chk1 when phosphorylated at other sites. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification
WebMonoclonal Antibody for studying Chk1 (Ser345) phosphate. Cited in 778 publications. Validated for WB, IF, F. Available in 3 sizes. Highly specific and rigorously validated in …
WebFeb 13, 2024 · The protein levels of PPP2R2A, pChk1-S317, pChk1-S345, Chk1, WEE1 and pCDK1-Y-15 were analyzed by Western blotting. GAPDH was used as loading control. C , D MCF-7 and 16HBE cells were treated as ... ipf financeWebDec 19, 2011 · Notably, the dissociation of Chk1 from Chk1-S in DDR appeared dependent on Chk1 phosphorylation at S345/S317, because the Chk1(S345A/S317A) mutant did not dissociate from Chk1-S during camptothecin treatment (Fig. 3E). In DDR, Chk1 activation depends on ATR-mediated phosphorylation (7, 13, 14). ipff iiWebCHK1 is phosphorylated on Serine 345 (S345) in response to UV, IR and hydroxyurea (HU). CHK1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development and tumor suppression. Further, CHK1 is a key mediator in the DNA damage-induced checkpoint network. CHK1 is an evolutionarily conserved protein kinase that … ipf fiscalWebMar 14, 2024 · Alzheimer’s disease (AD) is the most common neurodegenerative disease with limited therapeutic strategies. Cell cycle checkpoint protein kinase 1 (Chk1) is a Ser/Thr protein kinase which is activated in response to DNA damage, the latter which is an early event in AD. However, whether DNA damage-induced Chk1 activation participates in the … ipff iiaWebDec 1, 2002 · We have developed a kinase assay for Chk1, and have shown that basal Chk1 kinase activity is increased in response to DNA damage and that this increase, but … ipffmWebCHK1 is phosphorylated on Serine 345 (S345) in response to UV, IR and hydroxyurea (HU). CHK1 plays an essential role in the mammalian DNA damage checkpoint, embryonic … ipf financingWebLY2603618 inhibited Chk1 autophosphorylation (S296 Chk1) and increased DNA damage-mediated Chk1 phosphorylation (S345 Chk1). In addition, LY2603618-treated lung cancer cells transitioned from LC3-I to LC3-II, a hallmark of autophagy. Blocking autophagy with chloroquine (CQ) further enhanced LY2603618's inhibitory effect on cell viability ... ipff loan