Pcmv-mcherry-gfp-lc3b
Splet29 vrstic · Ad-mCherry-GFP-LC3B是碧云天自行研发的重组腺病毒，感染后能够在靶细胞中有效表达红色荧光蛋白mCherry、绿色荧光蛋白 (GFP)和LC3B的融合蛋白，呈现明亮的 … SpletThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Pcmv-mcherry-gfp-lc3b
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Splet07. nov. 2015 · Expression fluorescentprotein could TZM-tfLC3Bcells follow- ing more than 50 passages which indicated cellline (data shown).Fig. TZM-tfLC3Bcells expressed LC3B carrying mCherry-EGFP tag schematicdiagram mCherry-EGFPflorescent-tagged LC3B. florescent-taggedLC3B undercontrol cytomegalovirus-immediateearly promoter (PCMV … SpletA： 一般做自噬流检测我们不建议细胞再带其他颜色的荧光，例如是做基因敲减，有三种解决方案：. 1）建议将目的基因shRNA构建在自噬载体上（该载体已经带有红绿荧光），那么进行自噬慢病毒感染即可，该方案的优点是只感染一次病毒，操作简便，缺点是重新 ...
SpletAd-mCherry-GFP-LC3B是碧云天自行研发的重组腺病毒，感染后能够在靶细胞中有效表达红色荧光蛋白mCherry、绿色荧光蛋白(GFP)和LC3B的融合蛋白，呈现明亮的红色和绿色荧光，可以用于细胞自噬的检测。 自噬小体在与溶酶体融合过程中，溶酶体内的酸性环境会导致GFP荧光淬灭，这为追踪GFP-LC3B的细胞定位增加了难度。 mCherry是一种来自于蘑菇 … SpletpBABE-puro mCherry-EGFP-LC3B载体图谱质粒图谱、序列、价格、抗性、测序引物、大小等基本信息_生物风载体 pBABE-puro mCherry-EGFP-LC3B 价格： 1200元 联系方式： I47-825O-882O 相关技术服务： 质粒构建 基因合成 质粒大提 立刻购买 买家导航 载体基本信息 载体图谱质粒图谱和多克隆位点信息 载体简介 载体序列 LOCUS pBABE-puro mCherry …
SpletGFP-LC3单荧光和mCherry-GFP-LC3双荧光指示系统 自噬形成时，GFP-LC3或mCherry-GFP-LC3融合蛋白转移至自噬体膜，在荧光显微镜下形成多个明亮的绿色或黄色荧光斑点。 当自噬溶酶体形成后，酸性环境使GFP荧光淬灭，而mCherry荧光不受影响，自噬溶酶体呈现红色荧光。 因此，可以通过LC3荧光指示系统来监测自噬流。 LC3自噬双标系统追踪自噬流 … Splet02. jun. 2011 · A tandem fusion of mCherry and GFP is fused to LC3B (one of the several members of the mammalian LC3 family) to make a pH-sensitive sensor that is used to …
SpletmCherry-GFP-LC3B腺相关病毒 四．维真自噬双标系统的工作原理 未发生自噬的细胞及含有自噬体的细胞中，由于mCherry与GFP共同表达，细胞呈现黄色荧光。 当自噬体与溶酶体融合形成自噬溶酶体后，酸性的溶酶体环境使酸敏感的GFP荧光淬灭，而mCherry不受影响，进而使自噬溶酶体呈现红色荧光。 因此，红色荧光可指示自噬溶酶体形成的顺利程度。 红 …
SpletTo further analyze autophagy flux, we used L6 cells stably expressing fluorescent eGFP-mCherry-LC3B and conducted live time-lapse imaging every 1 hr up to 24 h after iron treatment. hemoglobin over hematocritSpletFluorescence resonance energy transfer (FRET) between fluorescent proteins (FPs) is a powerful tool to investigate protein-protein interaction and even protein modifications in living cells. Here, we analyze the E (0)GFP-mCherry pair and show that it can yield a reproducible quantitative determination of the energy transfer efficiency both in ... hemoglobin percentage in antenatal women pdfSplet07. nov. 2015 · Expression fluorescentprotein could TZM-tfLC3Bcells follow- ing more than 50 passages which indicated cellline (data shown).Fig. TZM-tfLC3Bcells expressed LC3B … lanett high school districtSpletDescription. pCMV-mCherry-GFP-LC3B is a plasmid developed by Biyuntian to express the red mCherry and green GFP double-labeled human LC3B fusion protein in mammalian cells. This plasmid contains the CMV promoter and is kanamycin resistant. After transfection, it can efficiently express the fusion protein of LC3B with the double label of red ... lanett whiteSpletPrice:888.00RMB. •. Purpose. Express Human LC3B protein with N-terminal mCherry and GFP tag in mammalian cell. •. Depositor. Beyotime. •. Publication. hemoglobin oximetrySpletpBABE-puro mCherry-EGFP-LC3B: microtubule-associated protein 1 light chain 3 beta (Homo sapiens) Debnath Add to Cart 24919: pCMV-myc-LC3 (Atg8) LC3 (Homo sapiens) ... pCMV 3xFLAG-LC3B G120A: Microtubule-associated proteins 1A/1B light chain 3B (Homo sapiens) Ketteler Add to Cart 123094: pCMV 3xFLAG-LC3B G120 ... lanett thyroid medicationhttp://www.app17.com/c145959/products/d11302689.html hemoglobin perth